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Update: 09 April 2021 21:43 STI
Bristol [UK]April 09 (ANI): Scientists at the tobacco company Imperial Brands used the ToxTracker suite of in vitro stem cell-based tests from Toxys that provide mechanistic insight into the potentially DNA-damaging properties of chemicals and have compared sample vape e-liquid and their aerosols with combustible cigarette smoke.
Imperial scientists are the first to publish results using the ToxTracker system for the evaluation of vape e-liquids and aerosols, and it’s part of the company’s ongoing research into the harm reduction potential of vaping. tobacco from next-generation products (NGP) such as vapes.
The assays help assess how product samples may impact cellular function in six reporter cell lines, detecting molecular signs indicative of potential damage in the form of oxidative stress, DNA and protein damage, as well as activation of the p53 gene which has a role in cell cycle regulation and tumor suppression.
The results, peer-reviewed and published in the journal Mutagenesis, showed that under the test conditions, undiluted vaping e-liquids and their aerosol extracts exhibited entirely absent or significantly reduced indications of potency. DNA damage in cells, compared to combustible cigarette smoke. .
“Overall, the data from our latest study adds to the weight of scientific evidence demonstrating that vaping products offer significant harm reduction potential compared to combustible cigarettes,” says Lukasz Czekala, senior preclinical toxicologist at Imperial. Brands and lead author of the article.
Result of the sequence
After calibrating and validating the system to ensure that the main e-liquid components Propylene Glycol (PG) and Vegetable Glycerin (VG) were compatible with the ToxTracker suite, a selection of pure myblu e-liquids vape and their aerosol extracts were compared to smoke samples. of the combustible cigarette of reference 1R6F. The results showed that:
– Vapor aerosols trapped in a buffer solution did not induce a response in any of the six cell lines.
– Undiluted flavored e-liquids (tested down to 1%) induced both oxidative stress reporters, but this was considered an effect of osmolarity (a measure of solution concentration) caused by PG /VG in an in vitro test environment.
-Nicotine content did not affect responses: tobacco-flavored e-liquid with 1.6% freebase nicotine, 1.6% nicotine salt, or no nicotine produced the same results. =
-In addition, nicotine (tested alone) only produced an oxidative stress response at levels that would be a concentration over 40,000 times greater than that found in the blood during typical smoking.
Dr. Fiona Chapman, preclinical toxicologist and corresponding author, adds that “ToxTracker is fast, sensitive and can provide greater mechanistic resolution than existing next-generation product (NGP) management DNA damage assays, such as microkernel and Ames tests.
She continued, “Our adoption of this state-of-the-art in vitro suite reinforces our commitment to using advanced cell-based assays that adhere to the principles of Toxicity Testing in the 21st Century (TT21C). It also helps to reduce industry’s reliance on in vivo (animal) testing experiments.” (Imperial does not test any products on animals: see our position here.)
“This paper adds to the established body of scientific evidence that shows that vaping products, when manufactured to high quality and safety standards, have significant potential to reduce the harms of tobacco compared to continuation of smoking,” says Dr. Grant O’Connell, head of tobacco harm reduction. Science at Imperial Brands.
“We appreciate society’s ongoing concerns about the health risks of smoking and are committed to undertaking high-quality research into nicotine products that are potentially less harmful than combustible tobacco for adult smokers.” (ANI)
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